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Advanced Light Microscopy Techniques (Sept. 22nd-24th, 2013)


Organizer:
Alexander Dammermann

Date

September, 22nd-24th, 2013

IMP lecture hall and MFPL seminar rooms

Poster

Background

Research in any area of cell biology including chromosome dynamics is heavily reliant on light microscopy to visualize structures and processes within the cell. This workshop provides an introduction to the theory and practice of light microscopy, through a series of lectures and seminars by experts in the field.

Speakers

Peter Carlton (Kyoto University)

Alexey Khodjakov (Wadsworth Center, NY)

Paul Maddox (IRIC, Montréal)

Iva Tolic-Norrelykke (MPI Dresden)

Clare Waterman (NIH)

Contents

The workshop will start with introductory lectures covering the fundamentals of microscopy, followed by research presentations of the invited speakers illustrating how microscopy can be applied to real-life biological questions. In addition, there will be round-table discussions with one visiting speaker per ~ 4 students, in which students have the opportunity to present their projects and get advice on how microscopy could be applied to their work.

All lectures will be open to everyone on campus, only round-table discussions will be limited to DK and associated students.

Program

Sunday, September 22nd, 2013

10.30h Introduction to optics, basic microscope design, phase contrast and DIC
Pawel Pasierbek
(IMP/IMBA)

14.00h Fluorescence, confocal microscopy, digital imaging
Paul Maddox (UNC, Chapel Hill, USA)

16.30h F-techniques: FRAP, FRET, etc.
Ivan Yudushkin (MFPL)

17.30h Superresolution microscopy
Kareem Elsayad (CSF)

 

Monday, September 23rd, 2013

09.00h Motor proteins caught in the act: single-molecule observations in vivo
Iva Tolić-Nørrelykke (MPI Dresden, DEU)

10.00h 3D Structured Illumination Microscopy (3D-SIM) and its application to chromosome structure
Peter Carlton (Kyoto University, JPN)

11.00h Cooperative mechanisms of mitotic spindle assembly
Alexey Khodjakov (Wadsworth Center, Albany, USA)

 

Tuesday, September 24th, 2013

10.00h Defining the cell migration molecular clutch using quantitative fluorescence microscopy
Clare M. Waterman (NIH, Bethesda, USA)

11.00h Imaging epigenetic dynamics at centromeres
Paul Maddox (UNC, Chapel Hill, USA)

Epilogue

The workshop was a great success - local microscopy experts introduced participants from the whole campus to the basic principles of light microscopy and presented various microscopy techniques. In the following hands-on-seesions, our invited speakers shared their experience with specific microscopy techniques and discussed potential applications with our students.

 

Opening

Sunday morning, Alex Dammermann opened the workshop.

 

Pawel

Pawel Pasierbek, our local microscopy expert, introduced participants to the basic principles of light microscopy with the help of lots of demonstration material.

 

lunch

The joint Sunday lunch was the first opportunity for speakers and participants to meet in a relaxed atmosphere.

 

processing

Paul Maddox explaining image processing during his talk on "Fluorescence, confocal microscopy, digital imaging"

 

student introduction

Our students introduced invited speakers to the audience (Jasmin Bassler and Iva Tolić-Nørrelykke)

student introduction_

(Swastika Sanyal and Alexey Khodjakov)

 

hands on AK

 

hand on IT

 

hands on

The hands-on-sessions completed the workshop.

MFPL

 

 

UniVie

 

 

GMI

 

 

IMBA Logo

 

 

FWF